Protein Labeling Reagents
Vector Laboratories NEUROBIOTIN Tracer
NEUROBIOTIN Tracer (SP-1120) is an amino derivative of biotin that can be used as an intracellular label for cells, particularly neurons. It is used for visualizing neural architecture and for the identification of gap junction coupling. Can be used in many types of preparations including in vivo, whole mounts, slice preparations, or cultured cells - Can be delivered by many routes such as intracellular electrodes, microinjection, cut-loading, or scrape-loading - Can be detected using avidin or streptavidin systems with either chromogenic or fluorescence visualization methods
Sartorius INCUCYTE MOUSE IGG2A FABFLUOR
IncuCyte® Mouse IgG2a FabFluor-488 Antibody Labeling Reagent for Live-Cell Immunocytochemistry
Vector Laboratories Concanavalin A (Con A) Fluorescein 25 mg
Concanavalin A (ConA) recognizes α-linked mannose residues present as part of a "core oligosaccharide" in many serum and membrane-bound glycoproteins. At neutral and alkaline pH, Con A exists as a tetramer, but below pH 5.6, it dissociates into dimers of 52 kDa. Derivatizations can produce stable forms with dimeric structures. Con A requires calcium or manganese ions at each binding site, so avoid using buffers that bind calcium (such as phosphate) to dilute Con A since a gradual loss in activity may occur. This fluorescein-labeled Con A features a ratio of fluorophores to lectin protein that provides optimal staining (excitation 495 nm, emission 515 nm). Supplied as a solution essentially free of unconjugated fluorophores, it is preserved with sodium azide. The recommended inhibiting/eluting sugar is a mixture of 200 mM α-methylmannoside/200 mM α-methylglucoside.
Biotium EB succinimidyl ester
The amine reactive succinimidyl ester of EB (ethidium bromide) can be conjugated to peptides, proteins, drugs, polymeric materials and biomolecules with primary amine groups. The conjugates are expected to be essentially nonfluorescent until they are able to complex with nucleic acids, resulting in red fluorescence. The conjugates might be useful for studies of nucleic acid binding to various biomolecules, such as DNA-binding proteins. It is also possible that conjugates of other biomolecules might be capable of monitoring their transport into the nucleus. EB dye conjugates of solid or semisolid matrices, such as microspheres, magnetic particles or various resins, might be useful for the detection or affinity isolation of nucleic acids.
Medchemexpress LLC HY-15939 10mg Medchemexpress, 6-FAM SE CAS:92557-81-8 Purity:>98%
Medchemexpress, HY-15939 10mg 6-FAM SE CAS:92557-81-8 Purity:>98% Medchemexpress has over 10000 novel life-science reagents, reference compounds, APIs and natural compounds for laboratory and scientific use. Other quantity can also be offered.
Lumiprobe Cyanine5 NHS ester, 1263093-76-0, 25mg
Molecular formula: C36H42N3BF4O4, Molecular weight: 667.54, Appearance: dark blue solid, Solubility: very poorly soluble in water (0.19 mM = 127 mg/L), good in polar (DMSO, DMF) and chlorinated (DCM, chloroform) organic solvents; Amine-reactive red emitting fluorescent dye for the labeling of proteins/peptides and amino-oligonucleotides. For the labeling of sensitive proteins, use water-soluble sulfo-Cyanine5 NHS ester.
STEMCELL Technologies GloCell™ Fixable Viability Dye Violet 540, Size: 100 Tests
GloCell™ Fixable Viability Dye Violet 540 is a fluorescent cell viability dye for staining live/dead mammalian cells in applications such as flow cytometry. The dye irreversibly binds intracellular and cell surface amine groups and can be utilized prior to fixation, permeabilization, or cryopreservation. Cells with compromised plasma membranes become permeable to the GloCell™ dye, resulting in a greater fluorescence compared to live cells in a cell viability assay.GloCell™ Fixable Viability Dye Violet 540 is excited by the violet laser at 405 nm and it has a fluorescence emission maximum of 540 nm that can be detected using the 525/50 band pass filter used to detect AmCyan.
Vector Laboratories Biotin (Long Arm) Hydrazide, 50 mg
Specialty Products. Biotin (Long Arm) Hydrazide reacts with aldehyde groups and thus can be used to label carbohydrate groups present in a wide variety of macromolecules. The spacer arm in this product allows optimal accessibility of avidin or streptavidin conjugates to biotin. Incorporation of Biotin (Long Arm) Hydrazide involves a two-step process. First, the saccharide is treated with periodate (not included) that produces aldehyde groups on sugars containing unsubstituted vicinal hydroxyl groups (these are usually present at the non-reducing termini of the oligosaccharide). Next, Biotin (Long Arm) Hydrazide reacts with the aldehyde groups to produce a stable linkage between the saccharide and biotin. This reagent can also be used to couple biotin to carboxyl groups on proteins and other molecules using a carbodiimide coupling method, and to specifically label RNA with biotin at the 3' end.
Enzo Life Sciences CYTO-ID Red long-term cell tracer kit (1Kit)
The CYTO-ID Red Long-Term Cell Tracer Kit uses proprietary noncovalent cell labeling technology to stably incorporate a red fluorescent dye containing hydrophobic aliphatic chains into the cell membrane’s lipid bilayer. The dye may be loaded into cells by following the included protocol. The labeling buffer is isotonic for mammalian cells and contains no detergents or organic solvents. The appearance of labeled cells may vary depending upon the cell type from uniformly bright to punctuate. This difference is thought to relate to the extent of membrane internalization occurring after cell labeling. The CYTO-ID Red Tracer Dye fluorescence is independent of pH within normally encountered physiologic ranges and fluorescence intensity per cell is typically unaffected by the ultimate pattern of dye distribution. Applications: Flow Cytometry, Fluorescence microscopy, Fluorescent detection .